Used to evaluate the entrapment efficiency and loading of ICG.

Used to detect the emission spectra of each sample.

Used to evaluate cellular uptake ability.

Used to investigate the morphology and structure of the nanoparticles.

Talos F200X G2 (S)TEM

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Used as a carrier for the nanoparticles.

Used as a component of the phase-transition nanoparticles.

Used as a chemotherapeutic drug loaded into the nanoparticles.

Used as a photo/sonosensitizer loaded into the nanoparticles.

Used for sonication in the preparation of nanoparticles.

Used to capture contrast-enhanced ultrasound (CEUS), and B-mode images.

Used to obtain and analyze PA-mode signals of the samples.

Used to determine cell viability.

Used to analyze apoptosis and CRT expression on the cell surface.

Used to detect the release of HMGB1 into the cell culture supernatant and its translocation to the cytoplasm.

Used to quantify the secretion of ATP into the supernatant.

Used as an indicator of fluorescence to detect the generation of intracellular ROS.

Used to assess LDH release.