研究目的
Developing a fast, convenient, and accurate colorimetric probe for the detection of adenosine in biological samples.
研究成果
The creatinine-Ag+/AuNPs system provides a rapid, selective, and sensitive method for adenosine detection, with potential applications in clinical diagnostics, especially for cancer diagnosis. The use of RGB values for quantification offers a simple, instrument-free approach suitable for resource-limited settings.
研究不足
The study's applicability in remote areas is highlighted, but the reliance on specific software (Microsoft PowerPoint) for RGB analysis may limit accessibility. The sensitivity and selectivity, while good, may not match some aptamer-based methods.
1：Experimental Design and Method Selection:
The study utilized a synergistic coordination chemistry approach on gold nanoparticles (AuNPs) for adenosine detection.
2：Sample Selection and Data Sources:
Adenosine samples were prepared in H2O, and bovine serum samples were used for practical application testing.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM, T20), UV-Vis spectrometer (Cary 60, Agilent), OHAUS Starter for pH adjustment, and various chemicals including gold(III) chloride trihydrate and sodium citrate dehydrate.
4：Experimental Procedures and Operational Workflow:
Synthesis of 13 nm AuNPs, preparation of the creatinine-Ag+/AuNPs system, and application for adenosine detection. Quantitative determination was achieved using the eyedropper function in Microsoft PowerPoint for RGB value analysis.
5：Data Analysis Methods:
UV-Vis spectra analysis and RGB value comparison for quantitative adenosine detection.
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UV-Vis spectrometer
Cary 60
Agilent
Used to measure the absorption spectra of the samples.
Cary 60 UV-Vis Spectrophotometer
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Transmission electron microscopy
T20
Used to obtain the morphological features of AuNPs.
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pH meter
OHAUS Starter
OHAUS
Used to adjust the pH of the testing system.
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Gold(III) chloride trihydrate
Energy Chemical Reagent Company
Used in the synthesis of AuNPs.
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Sodium citrate dehydrate
Energy Chemical Reagent Company
Used in the synthesis of AuNPs.
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