研究目的
To develop a manufacturing process for sheet-based stacked scaffolds (SSCs) using laser-cutting (LC) for tissue engineering applications, focusing on Polycaprolactone/Hydroxyapatite (PCL/HA) composite material.
研究成果
The LC process is a fast, reproducible, and flexible method for generating PCL/HA sheets for tissue engineering. It allows for the creation of scaffolds with interconnecting pores, facilitating cell seeding and analysis. The method supports cell adherence, growth, and differentiation, making it suitable for functional tissue constructs.
研究不足
The accuracy of the cutting process is dependent on the applied laser power. Structures thinner than 125 μm are not reliably stable. The process is limited to PCL/HA ratios between 0% and 50% HA.
1：Experimental Design and Method Selection:
Developed a manufacturing process for SSCs based on LC using PCL/HA composite material.
2：Sample Selection and Data Sources:
Used PCL/HA foil for cutting sheets, human mesenchymal stem cell line SCP-1 for cell culture.
3：List of Experimental Equipment and Materials:
Zing 24 laser cutter (Epilog), Zeiss Axio Observer.Z1 microscope, Mach1 mechanical tester, SEM Lyra 3 system.
4：Experimental Procedures and Operational Workflow:
Cut single sheets from PCL/HA foil, stacked to scaffolds, cultured SCP-1 cells on scaffolds, assessed cell growth and differentiation.
5：Data Analysis Methods:
Measured deviations between designed templates and resulting sheets, analyzed cell growth and differentiation.
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