1：Experimental Design and Method Selection:

The study employed a CO2 laser engraver system to inscribe patterns on soda lime glass slides, creating microfluidic channels with interconnected micro-cracks. The laser parameters (power and scanning speed) were varied to control the micro-crack formation.

2：Sample Selection and Data Sources:

Soda lime glass slides were used as substrates. The effect of laser parameters on crack patterns was characterized using optical microscopy.

3：List of Experimental Equipment and Materials:

CO2 laser engraver system (PLS6MW, Universal Laser Systems, Inc.), soda lime glass slides (GOLD SEAL? Micro Slide), Evans Blue solution, silica spheres, iron oxide micro-particles, silver nanoparticles, magnetic polystyrene bead solution.

4：Experimental Procedures and Operational Workflow:

The glass slides were irradiated with a single laser beam scan at varying power levels and scanning speeds to create microfluidic channels. The wicking and filtering properties were then characterized by measuring the average wicking speed of an aqueous dye and the filtering capability of the channels.

5：Data Analysis Methods:

The wicking speed was measured using a hand-held stopwatch. The filtering performance was analyzed by comparing the difference of scattered light intensity between inlet and outlet solutions using a particle size analyzer.