研究目的
To develop a quick, efficient, and cost-effective method for in-situ detection of live, dead, and antibiotic-resistant bacteria using silver nanorods array sensor and a mobile app.
研究成果
The developed silver nanorods array sensor combined with a mobile app provides a facile, inexpensive, and scalable method for the in-situ detection of live, dead, and antibiotic-resistant bacteria, with potential applications in clinical diagnosis.
研究不足
The study is limited to the detection of bacteria that produce H2S gas and may not be applicable to all types of bacteria. The sensitivity and selectivity of the AgNRs array sensor need further optimization for broader application.
1:Experimental Design and Method Selection:
The study utilized silver nanorods (AgNRs) arrays fabricated by glancing angle deposition technique for detecting H2S gas produced by bacteria.
2:Sample Selection and Data Sources:
E.coli, P. aeruginosa, B. subtilis, and S. aureus were used as model organisms.
3:List of Experimental Equipment and Materials:
Silver nanorods array sensor, bacterial cultures, and a mobile app 'Colorimetric Detector'.
4:Experimental Procedures and Operational Workflow:
Exposure of AgNRs array to bacterial cultures and observation of changes in color and wettability.
5:Data Analysis Methods:
Quantification of changes in wettability and color using water contact angle and optical darkness ratio (ODR) respectively.
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