1：Experimental Design and Method Selection:

The study involved the non-covalent coupling of C60 to a porphyrin-derived metal-organic framework (PCN-224) to form a C60@PCN-224 nanohybrid. The photoelectrochemical properties of this nanohybrid were investigated for immunosensing applications.

2：Sample Selection and Data Sources:

The study used S100 calcium-binding protein B (S100B) as a proof-of-concept analyte to demonstrate the application of the C60@PCN-224 nanohybrid in photoelectrochemical immunosensing.

3：List of Experimental Equipment and Materials:

Materials included C60, PCN-224, Nanobodies (Nb82 and Nb9), and various chemicals for the preparation of the nanohybrid and immunosensor. Equipment included a CHI 600e electrochemical workstation, Xe lamp, TEM, SEM, UV?vis spectrophotometer, and fluorescence spectrometer.

4：Experimental Procedures and Operational Workflow:

The C60@PCN-224 nanohybrid was prepared and characterized. The immunosensor was fabricated by modifying an ITO electrode with the nanohybrid, followed by conjugation with Nanobodies and detection of S100B.

5：0B. Data Analysis Methods:

5. Data Analysis Methods: The photocurrent response was measured under chopped light irradiation. The performance of the immunosensor was evaluated based on the photocurrent change in response to different concentrations of S100B.