研究目的
Investigating the spatiotemporal dynamics of laser-induced crystal precursors in aqueous solutions using particle image diffusometry (PID) for label-free visualization.
研究成果
The study successfully visualized the spatiotemporal dynamics of laser-induced crystal precursors in aqueous solutions using PID. It revealed the existence of loose aggregation domains of prenucleation clusters with viscosity comparable to honey, providing insights into the crystallization process.
研究不足
The study is limited by the resolution of PID analysis and the detection range of the measurement system. The method may not detect tightly connected prenucleation clusters in high-concentration solutions.
1：Experimental Design and Method Selection:
The study employs particle image diffusometry (PID) for the tracking-free evaluation of the diffusion-coefficient field from microscopy movie data.
2：Sample Selection and Data Sources:
Aqueous solutions of L-phenylalanine (L-Phe) and acetaminophen (Ace) in D2O were used.
3：List of Experimental Equipment and Materials:
An inverted optical microscope equipped with a sCMOS camera, a continuous-wave laser of 1064 nm, and an objective lens.
4：Experimental Procedures and Operational Workflow:
Laser irradiation into solutions was controlled to induce crystallization, and the microscopy movie data were sampled intermittently.
5：Data Analysis Methods:
PID analysis was applied to the microscopy movie data to evaluate the diffusion coefficient field.
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