研究目的
Investigating the expression and activity of ion transport-related molecules in cancer stem cells (CSCs) of esophageal squamous cell carcinoma (ESCC) to identify new chemotherapeutic targets.
研究成果
TRPV2 is involved in the maintenance of CSCs, and Tranilast, a specific inhibitor of TRPV2, shows promise as a targeted therapeutic agent against ESCC. pCLE is comparable to standard biopsies in detecting persistent/recurrent IM after endoscopic treatment of BORN, but larger studies are needed for confirmation.
研究不足
The study's findings need to be confirmed in a larger cohort of patients. The specificity and sensitivity of pCLE in detecting persistent/recurrent IM after endoscopic treatment of BORN require further validation.
1：Experimental Design and Method Selection
Cells with high expression of ALDH1A1 were sorted via FACS, and CSCs were generated using the sphere formation assay. Gene expression profiles of CSCs were examined using microarray analysis.
2：Sample Selection and Data Sources
CSCs derived from two types of esophageal cancer cell lines.
3：List of Experimental Equipment and Materials
FACS for cell sorting, microarray for gene expression analysis, RT-PCR for validation.
4：Experimental Procedures and Operational Workflow
Sorting cells with high ALDH1A1 expression, generating CSCs via sphere formation assay, analyzing gene expression profiles, validating TRPV2 mRNA upregulation, testing Tranilast cytotoxicity.
5：Data Analysis Methods
Microarray analysis for gene expression changes, RT-PCR for validation, cytotoxicity assays for Tranilast effects.
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