研究目的
To extract and characterize a water-soluble non-starch polysaccharide from red yeast rice (RYRP) using ultrasonic-assisted extraction method and evaluate its antioxidant activities and gastrointestinal-protective effect.
研究成果
The study successfully extracted a water-soluble non-starch polysaccharide from red yeast rice using UAE, optimized the extraction conditions, and characterized its structure. RYRP exhibited significant antioxidant activities in vitro and a gastrointestinal-protective effect in vivo, suggesting its potential applications in the pharmaceutical and functional food industries.
研究不足
The study focuses on the extraction and characterization of RYRP and its bioactivities, but the mechanisms underlying its antioxidant and gastrointestinal-protective effects require further investigation. Additionally, the scalability of the UAE method for industrial applications was not addressed.
1：Experimental Design and Method Selection:
Ultrasonic-assisted extraction (UAE) was used to extract RYRP from red yeast rice. Box–Behnken design (BBD) was employed to optimize the extraction parameters.
2：Sample Selection and Data Sources:
Red yeast rice was purchased from Sichuan Sino-Dandard Pharmaceutical Co., Ltd.
3：List of Experimental Equipment and Materials:
Ultrasonic cleaning machine (KQ5200DE, Kunshan Ultrasonic Co. Ltd., China), centrifuge (L-550, Xaingyi Centrifuge Instrument Co., LTD, China), and various chemicals for analysis.
4：Experimental Procedures and Operational Workflow:
The dried red yeast rice was ground and extracted with distilled water under various conditions. The supernatant was obtained by centrifugation, treated with α-amylase to break down starch, and then the polysaccharide was precipitated, deproteinized, decolorized, and analyzed.
5：Data Analysis Methods:
The extraction yield was analyzed by the anthrone-sulfuric acid method. Structural characterization was performed using SEM, FT-IR, HPLC, and HPGPC. Antioxidant activities were evaluated through DPPH, ABTS, ferrous ion chelating, and hydroxyl radical scavenging assays. The gastrointestinal-protective effect was assessed in GD model mice.
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