研究目的
Investigating the efficient photoinjection of megadalton molecules into intact plant cells using a femtosecond laser amplifier under moderate enzyme treatment conditions.
研究成果
The work successfully demonstrates the photoinjection of megadalton molecules into intact plant cells using a femtosecond laser amplifier and enzyme treatment, indicating potential for efficient gene transfer methods in plant molecular breeding.
研究不足
The study demonstrates the introduction of large molecules into plant cells but does not assess the expression of actual genes or their functionality post-introduction.
1：Experimental Design and Method Selection:
The study employed a femtosecond laser amplifier for photoporation under moderate enzyme treatment conditions to introduce large molecules into plant cells.
2：Sample Selection and Data Sources:
Tobacco BY-2 cells were used as the model system.
3：List of Experimental Equipment and Materials:
A regeneratively amplified fs Ti:sapphire laser system, confocal fluorescence microscope, and enzyme solutions were used.
4：Experimental Procedures and Operational Workflow:
Cells were treated with mannitol and enzymes, followed by laser irradiation and fluorescence imaging to assess molecular introduction.
5：Data Analysis Methods:
Differential fluorescence intensities were measured to quantify the level of introduced molecules.
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