研究目的
Developing a simple and convenient fluorescence method for the detection of uric acid (UA) based on Ag-doped carbon quantum dots (Ag-CQDs) with high sensitivity and selectivity.
研究成果
The Ag-CQDs-based sensor demonstrated high sensitivity, selectivity, and rapid response for UA detection, with a wide linear range and low detection limit. It was successfully applied to detect UA in human urine samples, showing great potential for clinical diagnosis.
研究不足
The study focuses on the detection of UA in human urine samples, and the interference from other substances in more complex matrices was not extensively studied. The practical application in clinical settings requires further validation.
1：Experimental Design and Method Selection:
The Ag-CQDs were prepared by one-step hydrothermal treatment of ethylenediamine and silver nitrate in aqueous solution. The fluorescence intensity of Ag-CQDs was measured in the presence of UA to detect its concentration.
2：Sample Selection and Data Sources:
Human urine samples were used to test the practical application of the Ag-CQDs for UA detection.
3：List of Experimental Equipment and Materials:
F-4600 fluorescence spectrophotometer, ESCALAB 250XI electron spectrometer, HT-7700 transmission electron microscopy, PerkinElmer FTIR spectrophotometer, U-2910 UV absorption spectrophotometer, Edinburgh Instruments FLS920 spectrofluorimeter.
4：Experimental Procedures and Operational Workflow:
The Ag-CQDs were synthesized, characterized, and then used to detect UA in samples. The fluorescence intensity was measured before and after adding UA.
5：Data Analysis Methods:
The fluorescence quenching extent was analyzed to determine the concentration of UA, with a linear relationship established between the fluorescence intensity ratio and UA concentration.
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spectrofluorimeter
FLS920
Edinburgh Instruments
Measuring quantum yields
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fluorescence spectrophotometer
F-4600
Hitachi
Measuring fluorescence intensity
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electron spectrometer
ESCALAB 250XI
Thermo
X-ray photoelectron spectroscopy data collection
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FTIR spectrophotometer
PE-983
PerkinElmer
Fourier-transform infrared spectrum recording
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UV absorption spectrophotometer
U-2910
Hitachi
UV absorption spectrum recording
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transmission electron microscopy
HT-7700
Tokyo
Transmission electron microscopy imaging
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