研究目的
To develop a fluorescent nanosensor based on colloidal quantum dots CdSe/ZnS modified with mercaptoacetic acid for the determination of reduced glutathione, a non-protein compound important in protection against oxidative stress.
研究成果
The developed fluorescent nanosensor system allows for sensitive detection of reduced glutathione in aqueous solutions in the concentration range of 10–1000 μM, with a detection limit of 3 μM. This system holds promise for developing a fast, sensitive, and selective method for determining reduced glutathione in blood plasma, which is crucial for medical diagnosis.
研究不足
The detection limit of the proposed method is 3 μM, and the greatest sensitivity occurs in the concentration range of 25–250 μM. At lower and higher concentrations, sensitivity decreases. The system requires an incubation time of 25–30 min for optimal results.
1：Experimental Design and Method Selection:
The study involved the development of a fluorescent nanosensor using colloidal quantum dots CdSe/ZnS modified with mercaptoacetic acid. The principle of operation is based on the restoration of 'turned-off' fluorescence under the influence of reduced glutathione.
2：Sample Selection and Data Sources:
The samples included aqueous solutions of reduced glutathione with concentrations ranging from 0 to 1000 μM.
3：List of Experimental Equipment and Materials:
CdSe/ZnS-based colloidal quantum dots modified by mercaptoacetic acid, SFF-2 Fluoran spectrophotometer, Malvern Zetasizer Nano ZS nanoparticle size analyzer.
4：Experimental Procedures and Operational Workflow:
The process involved purifying quantum dots, quenching their fluorescence with methyl viologen, and then restoring fluorescence by adding reduced glutathione. Fluorescence intensity was measured at fixed emission wavelengths.
5：Data Analysis Methods:
The dependence of fluorescence intensity on the concentration of reduced glutathione was analyzed to construct a calibration curve.
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