研究目的
To measure the size of Daphnia pulex under freely-swimming and stress-free conditions using digital holography (DH) and compare the results with conventional manual eye measurement methods.
研究成果
The study successfully demonstrated the size measurement of Daphnia pulex using incoherent Gabor digital holography (DH). The results agreed well with those obtained by conventional manual eye measurement, with a mean difference of 38 μm. The method is promising for fieldwork, though further improvements in accuracy and automation are needed.
研究不足
The accuracy of the size measurement could be further improved with more sophisticated image-processing software and a camera with more pixels and lower noise. The release of the shutter and the post-focusing were performed manually, indicating a need for fully automatic measurement for fieldwork.
1：Experimental Design and Method Selection:
The study used low-coherence Gabor digital holography (DH) for size measurement of freely swimming Daphnia pulex. The method leverages the post-focusing ability of DH to capture three-dimensional images of moving targets.
2：Sample Selection and Data Sources:
Daphnia pulex in water in a plastic container was used as the sample.
3：List of Experimental Equipment and Materials:
A green LED light source, a plastic container for the sample, a CMOS image sensor, and optics for magnification were used.
4：Experimental Procedures and Operational Workflow:
The interference image was captured by a CMOS image sensor through magnification optics. The hologram was processed to reconstruct the image of the Daphnia pulex, followed by image processing to measure the body size.
5：Data Analysis Methods:
The body size was measured by counting bright pixels on the principal axis of the binary image obtained from the processed hologram.
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