研究目的
To develop efficient photothermal nanomaterials with high NIR-II absorbance for imaging-guided photothermal therapy in the second near-infrared window.
研究成果
The as-synthesized 9T-GQDs manifest a uniform morphology with smaller size distribution and good water-solubility and excellent biocompatibility. Both in vitro and in vivo results show that the as-synthesized GQDs could efficiently kill tumor cells and inhibit the tumor grown at a 1064 nm laser with a power density of 1.0 W/cm2. The as-synthesized 9T-GQDs also exhibit obviously NIR imaging of tumor site in living mice, suggesting the great probability of 9T-GQDs for NIR imaging-guided PTT.
研究不足
The study focuses on the synthesis and application of 9T-GQDs for photothermal therapy and imaging in the NIR-II window, but does not explore the long-term toxicity or degradation pathways of these nanomaterials in vivo.
1：Experimental Design and Method Selection:
The 9T-GQDs were synthesized via a one-step solvothermal process using phenol molecules as single precursors and hydrogen peroxide as oxidant under an external high magnetic field (HMF) with an intensity of 9 Tesla. The HMF is used to control the concentration of dissolved oxygen in the reaction system and the accordingly formation of superoxide radicals during the decomposition of phenol molecules, leading to the formation of GQDs with abundant C=O bonds and larger conjugated system.
2：Sample Selection and Data Sources:
Phenol (0.067 g) was dissolved in a solution of 20 mL acetone. After intense sonication for 30 min, 1.0 mL of H2O2 (30%) was slowly added into the solution.
3：067 g) was dissolved in a solution of 20 mL acetone. After intense sonication for 30 min, 0 mL of H2O2 (30%) was slowly added into the solution.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Hydrogen peroxide (H2O2, 30%), acetone (C3H6O), phenol (C6H6O), dimethyl sulfoxide (DMSO) and N,N-dimethylformamide (DMF) were used as received without further purification. The water used in all experiments was of Millipore Milli-Q grade.
4：Experimental Procedures and Operational Workflow:
The solution was then treated ultrasonically for 10 min and transferred into a 25 mL Teflon-lined stainless autoclave chamber. The precursor solution was heated to and maintained at 200 °C with an external high magnetic field with an intensity of 9T. After 20 h, the solution was cooled naturally to room temperature. The resultant product was purified with repeated centrifugation at a speed of 12000 rpm for 20 min and redispersion in water for three cycles. Finally, the aqueous dispersion of 9T-GQDs and 0T-GQDs were dialyzed for 3 days at room temperature.
5：9T. After 20 h, the solution was cooled naturally to room temperature. The resultant product was purified with repeated centrifugation at a speed of 12000 rpm for 20 min and redispersion in water for three cycles. Finally, the aqueous dispersion of 9T-GQDs and 0T-GQDs were dialyzed for 3 days at room temperature.
Data Analysis Methods:
5. Data Analysis Methods: The optical properties of the as-synthesized GQDs were studied using UV-vis-NIR Spectrometer (Lambda 365, PerkinElmer Co., USA). The photothermal experiments were conducted using a NIR laser with a wavelength of 1064 nm and power density of 1.0 W cm?2 (Lasever Inc, Ningbo, China).
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