研究目的
To develop and evaluate two methods for extracting the reduced scattering and absorption coefficients from spatially resolved diffuse reflectance spectroscopy (SRDRS) data of colon tissue using lookup tables of simulated diffuse reflectance.
研究成果
The SC-LUT method, which leverages both spatial and spectral information, significantly reduces the error in extracting optical properties compared to the SR-LUT method. This approach demonstrates the potential for more accurate characterization of colon tissue using SRDRS.
研究不足
The study was limited by the use of the Henyey-Greenstein phase function in MC simulations, which may not fully account for sub-diffuse effects. Additionally, the SC-LUT method averages optical properties over a larger volume, potentially reducing volume resolution.
1：Experimental Design and Method Selection:
The study involved the development of two inverse methods for extracting optical properties from SRDRS data using lookup tables (LUTs) populated with Monte Carlo (MC) simulated diffuse reflectance. The methods were evaluated using experimental measurements of liquid tissue phantoms and ex-vivo human colon tissue.
2：Sample Selection and Data Sources:
Liquid phantoms were prepared with polystyrene spheres and hemoglobin in deionized water. Ex-vivo human colon tissue samples were measured within 30 minutes after resection.
3：List of Experimental Equipment and Materials:
A custom multi-pixel silicon SRDRS sensor, a 300 W Xenon lamp coupled to a monochromator, transimpedance amplifier (TIA) arrays, and a spectrophotometer were used.
4：Experimental Procedures and Operational Workflow:
The MEOS probe was used to measure reflectance from liquid phantoms and human colon tissue. The optical properties were extracted using the developed inverse methods.
5：Data Analysis Methods:
The performance of the inverse methods was quantified by the mean absolute value percent error between the theoretical and extracted values of the optical properties.
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