研究目的
To develop a simple-structured upward open-channel microbial fuel cell (UOC-MFC) for in-situ detecting oil presence in a real-time self-sustained mode, addressing the shortcomings of existing oil leakage detection approaches like low-sensitivity, environment-vulnerability, and high cost and energy consumption.
研究成果
The UOC-MFC biosensor demonstrated great potential for in-situ monitoring of oil leakage with advantages of simple structure, low cost, easy deployment and operation, fast response, good selectivity, good self-sustainability and reusability, and low maintenance requirement. The integration of UOC-MFC with a PMS was theoretically able to support a miniature multimeter and voltage transmitter, enabling real-time oil monitoring in a self-sustained way.
研究不足
The study acknowledges the potential impact of biofouling on cathode and the need for periodic replenishment of substrate to the electrolyte solutions. Additionally, the response time of the UOC-MFC sensor was longer than those of MFC biosensors that depend on the sharp change of anaerobic microbes in the anode.
1：Experimental Design and Method Selection:
A tubular UOC-MFC sensor was fabricated using 3-D printing technology, with a circular cathode floating on the water-air interface. The sensor's response to different engine oil (EO) shocks was measured by the external potential across a constant load, internal resistance, and response time.
2：Sample Selection and Data Sources:
Engine oil (EO) was used to simulate real-world oil leakage accidents. The potential reading of the UOC-MFC sensor was recorded every 10 min.
3：List of Experimental Equipment and Materials:
The UOC-MFC sensor consisted of an anode submerged in electrolyte solution, an open-channel circular cathode floating on water surface, and the nutrient electrolyte solution. Equipment included a 3D printer, carbon cloth for anode and cathode, platinum as ORR catalyst, and a voltage recorder.
4：Experimental Procedures and Operational Workflow:
The sensor was inoculated and its reading stability was examined. Different amounts of EO were added to the contact reservoir surrounded by the cathode, and the sensor's response was recorded. The cathode was cleaned after each test to recover the voltage signal.
5：Data Analysis Methods:
The relationship between the signal variation of the UOC-MFC sensor and the oil amount was established. The polarization curve, power density curve, and inner resistance were plotted and calculated.
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