研究目的
To develop a minimally invasive platform for chemical sensing in interstitial fluid (ISF) using surface-enhanced Raman spectroscopy (SERS) integrated with microneedle arrays, focusing on pH sensing as a demonstration of the platform's capabilities.
研究成果
The plasmonic microneedle array SERS sensor was successfully developed and demonstrated to measure pH levels in solutions, agar gel skin phantom, and human skin in situ. The sensor is reversible, reproducible, and mechanically robust, offering a strategy for sensing a variety of relevant analytes in interstitial fluids.
研究不足
The study has not characterized the long-term stability and performance of the sensor arrays in animal models, and comments on the stability of the sensor to biofouling and any toxicity are not provided.
1：Experimental Design and Method Selection:
The study involved the fabrication of microneedle arrays from a commercial polymeric adhesive (NOA 65) and coating them with plasmonically active gold nanorods functionalized with 4-mercaptobenzoic acid (4-MBA) for pH sensing. The SERS activity was calibrated and tested in various environments including solutions, agar gel skin phantom, and human skin.
2：Sample Selection and Data Sources:
Human skin and agar gel skin phantom were used to test the sensor's performance in situ.
3：List of Experimental Equipment and Materials:
Norland Optical Adhesive (NOA) 65, gold nanorods (AuNRs), 4-mercaptobenzoic acid (4-MBA), Britton-Robinson buffers, Dulbecco’s phosphate buffered saline (DPBS).
4：Experimental Procedures and Operational Workflow:
Microneedle arrays were fabricated using a PDMS mold, treated with ozone, and then incubated with AuNRs and 4-MBA. The SERS activity was measured in different pH environments and after mechanical stress tests.
5：Data Analysis Methods:
The SERS spectra were analyzed by measuring the intensity ratio of the carboxylate peak to a reference peak to determine pH levels.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
