研究目的
Investigating the self-assembly of reconfigurable plasmonic diastereomers based on DNA nanotechnology and their chiroptical responses.
研究成果
The study successfully demonstrated the self-assembly of reconfigurable plasmonic diastereomers with up to three chiral centers, each controllable between left-handed and right-handed states. The near-field coupling of chiral centers significantly contributes to the overall CD signals, enabling the tuning of chiroptical outputs by changing the arrangement of chiral centers. This work provides a foundation for understanding and designing complex chiral plasmonic structures.
研究不足
The yield of AuNR tetramers was not as high as trimers, requiring a centrifuge concentration step for CD investigations. The study focuses on systems with up to three chiral centers, and the complexity increases with more centers.
1：Experimental Design and Method Selection:
The study employs DNA origami as a template for organizing gold nanorods (AuNRs) into chiral centers. Each chiral center can be switched between left-handed and right-handed states using DNA strand displacement reactions.
2：Sample Selection and Data Sources:
AuNRs of specific dimensions (12 nm × 42 nm) were synthesized and functionalized with thiolated DNA for assembly on DNA origami templates.
3：List of Experimental Equipment and Materials:
DNA origami templates, AuNRs, DNA strands for assembly and reconfiguration, agarose gel electrophoresis for purification, TEM for characterization, and CD spectrometer for optical measurements.
4：Experimental Procedures and Operational Workflow:
Assembly of AuNR dimers and trimers on DNA origami, purification by gel electrophoresis, TEM imaging, and CD spectral characterization. Reconfiguration of chiral centers was achieved by adding specific DNA fuel strands.
5：Data Analysis Methods:
CD spectra were analyzed to determine the chiroptical responses of the plasmonic diastereomers. Theoretical calculations using finite-element EM method (COMSOL multiphysics) were performed to simulate the optical properties.
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