研究目的
To develop a method for convenient and effective N-glycan detection by coupling condition controlled chemical derivatization with MALDI-TOF MS.
研究成果
The developed method provides a convenient and efficient approach for N-glycan detection in complex biological samples, with enhanced signal intensities and identification scales compared to direct analysis.
研究不足
The method's efficiency may be limited by the complexity of biological samples and the need for optimization of derivatization conditions for different types of N-glycans.
1：Experimental Design and Method Selection:
The method involves coupling chemical derivatization with MALDI-TOF MS for N-glycan detection.
2：Sample Selection and Data Sources:
N-glycans were released from asialofetuin and human plasma glycoproteins.
3：List of Experimental Equipment and Materials:
MALDI-TOF MS, 1-pyrenebutyric hydrazide (PBH), 2,5-dihydroxybenzoic acid (DHB), maltoheptaose (DP7), trifluoroacetic acid, asialofetuin, peptide-N-glycosidase F (PNGase F).
4：Experimental Procedures and Operational Workflow:
N-glycans were derivatized with PBH in methanol at 90°C for 60 min, then analyzed by MALDI-TOF MS.
5：Data Analysis Methods:
Data were analyzed by Data Explorer 4.5 software and GlycoWorkbench software.
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