研究目的
To develop a rapid, simple, and specific bioprobe for the detection of Escherichia coli O157:H7 in contaminated foods using amino-functionalized graphene quantum dots (af-GQDs) as immunofluorescence probes.
研究成果
The af-GQDs immunofluorescent probe demonstrated high sensitivity and specificity for detecting E. coli O157:H7, with a minimum detection limit of 100 cfu/mL. The method is simple, rapid, and cost-effective, offering a promising tool for food safety monitoring.
研究不足
The study acknowledges the challenge of nonspecific binding between the immunofluorescent probe and bacteria, which was mitigated using 1% BSA as a blocking agent. The detection limit, while low, may still require optimization for certain applications.
1：Experimental Design and Method Selection:
The study involved the preparation of af-GQDs via hydrothermal method and their characterization using HRTEM, AFM, UV-vis, fluorescence, and Raman spectroscopic techniques.
2：Sample Selection and Data Sources:
E. coli O157:H7 bacterial strains were obtained from China Center of Industrial Culture Collection.
3：List of Experimental Equipment and Materials:
Included HRTEM (JEOL, JEM-2100F), AFM (MFP-3D-BIO), UV-Vis spectrophotometer (TU 1901), and fluorescence spectrophotometer (Hitachi F-4600).
4：0). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Involved the synthesis of af-GQDs, their bioconjugation with antibodies, and the detection of E. coli O157:H7 using the prepared immunofluorescence probes.
5：Data Analysis Methods:
The specificity and sensitivity of the probes were evaluated through fluorescence microscopy and spectroscopy.
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