研究目的
To develop and evaluate two 55Co-labeled albumin-binding folate derivatives as potential PET agents for folate receptor imaging.
研究成果
The study successfully developed and evaluated two 55Co-labeled albumin-binding folate derivatives for PET imaging of folate receptor-positive tumors. Both compounds showed high radiochemical yields, excellent in vitro stability, and significant tumor uptake in vivo. The choice of chelator for 55Co can be manipulated to fine-tune the in vivo behavior of 55Co-labeled bioconjugates without sacrificing stability. These findings support the continued investigation of 55Co-labeled bioconjugates for use in nuclear medicine.
研究不足
The study's limitations include the inability of the 55Co-labeled radiofolates to distinguish between the FRα and FRβ isoforms, which could lead to false positives in oncologic imaging. Additionally, the lower effective molar activity of the 55Co-labeled radiofolates compared to previous studies may affect the results.
1：Experimental Design and Method Selection:
The study involved the synthesis and evaluation of two 55Co-labeled folate derivatives for PET imaging. The methodology included radiolabeling, in vitro stability tests, cell binding and internalization assays, and in vivo biodistribution studies in KB tumor-bearing mice.
2：Sample Selection and Data Sources:
The human cervical cancer KB cell line was used for in vitro studies, and female athymic nude mice implanted with KB cells were used for in vivo studies.
3：List of Experimental Equipment and Materials:
Equipment included an Agilent 1260 Infinity HPLC system, a Flow-RAM NaI detector, and a GNEXT PET/CT imaging scanner. Materials included 55Co, folate derivatives cm10 and rf42, and various chemicals for radiolabeling and stability tests.
4：Experimental Procedures and Operational Workflow:
The study involved radiolabeling folate derivatives with 55Co, assessing their in vitro stability in PBS and mouse serum, evaluating their uptake in KB cells, and conducting biodistribution and PET/CT imaging studies in mice.
5：Data Analysis Methods:
Data were analyzed using HPLC, radio-TLC, and gamma counting. PET/CT images were reconstructed and analyzed using specialized software.
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