研究目的
To develop a rapid, sensitive analytical method for the detection of ascorbic acid (AA) using carbon quantum dots (CQDs) synthesized by microwave irradiation.
研究成果
The CQDs/GCE sensor demonstrated wide linear ranges, high sensitivity, and good selectivity for AA detection, making it a promising tool for real sample analysis.
研究不足
The study focuses on the detection of AA and does not explore the simultaneous detection of other biomolecules in depth. The sensor's performance in highly complex biological matrices needs further validation.
1:Experimental Design and Method Selection:
CQDs were synthesized by microwave irradiation and electropolymerized on a glassy carbon electrode (GCE) to establish an electrochemical sensor for AA detection. Electrochemical behaviors were investigated by CV, DPV, and EIS.
2:Sample Selection and Data Sources:
AA was purchased from Lianxing Biotechnology (Tianjin), and other reagents were prepared with ultrapure water.
3:List of Experimental Equipment and Materials:
AMETEK PARSTAT 4000 electrochemical workstation, Nova NanoSEM 430, FEI Talos F200X, LX-400 centrifuge, microwave reactor, dialysis bags.
4:Experimental Procedures and Operational Workflow:
Synthesis of CQDs, preparation of CQD modified GCE, electrochemical characterization, and real sample analysis.
5:Data Analysis Methods:
The electrochemical response was analyzed using CV, DPV, and EIS techniques.
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FEI Talos F200X
Talos F200X
FEI
High-resolution electron microscopy and energy-dispersive spectrometer micrographs
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AMETEK PARSTAT 4000 electrochemical workstation
PARSTAT 4000
AMETEK
Electrochemical measurements
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Nova NanoSEM 430
Nova NanoSEM 430
FEI
Field emission scanning electron microscopy imaging
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LX-400 centrifuge
LX-400
Kylin-Bell Lab Instruments Co., Ltd.
Centrifugation of CQDs
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Microwave reactor
LG Electronics
Synthesis of CQDs
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Dialysis bags
Beijing Solarbio Science & Technology
Dialysis of CQDs
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