研究目的
This study compared photodynamic therapy (PDT) with glucose for vessel sclerosis in a rabbit ear model.
研究成果
PDT showed promising results in the sclerosis of small high ?ow vessels in a rabbit ear model, which were a challenge for glucose sclerosis. The safety of the PDT procedure seemed adequate, as only rare histological alterations were found in the study group.
研究不足
The rabbit ear model is not ideal because the rabbit ear is also the organ responsible for thermal regulation and, therefore, these vessels can dilate themselves at high temperature. It is not possible to directly transpose these results for a clinical trial.
1：Experimental Design and Method Selection:
The study was an open randomised study, approved by the Institutional Animal Care and Use Committee. Thirty-six ears of 18 rabbits were randomly divided into four groups for different treatments.
2：Sample Selection and Data Sources:
Healthy Norfolk rabbits (average weight 2 kg) were used.
3：List of Experimental Equipment and Materials:
Photogem, a haematoporphyrin derivative, was used as a photosensitiser. A thermal imager (Fluke FLK-Ti400 model) and a camera (model Sony DSC-H50) were used for image acquisition.
4：Experimental Procedures and Operational Workflow:
Injections of glucose or PS solutions were made into the ear central artery. After injections or during light exposure, manual compression of the marginal ear vein was performed. Images were taken before, immediately after, one day, and six days after the procedures.
5：Data Analysis Methods:
The percentage of length reduction of spider veins was analysed in digital photographs with Image J software. Thermal images were processed using MATLAB software.
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