研究目的
To overcome the limitations of flat-cleaved optical fibers in fiber photometry by exploiting the modal properties of tapered optical fibers for deeper and more versatile light collection in neural activity monitoring.
研究成果
Tapered optical fibers offer a significant advancement over flat-cleaved fibers for fiber photometry, enabling deeper and more versatile light collection in neural tissue. This technology facilitates simultaneous monitoring of neural activity across multiple brain regions and can be engineered for specific experimental needs.
研究不足
The study primarily focuses on the technical demonstration of TFs' capabilities in fiber photometry. The practical application in diverse biological systems and the long-term effects of TF implantation in neural tissue require further investigation.
1：Experimental Design and Method Selection:
Utilized tapered optical fibers (TFs) for light collection in neural tissue, comparing their performance with flat-cleaved fibers (FFs). Implemented two-photon scanning systems for fluorescence generation and collection.
2：Sample Selection and Data Sources:
Used brain slices from wild-type and Thy1-ChR2-EYFP mice, and in vivo experiments with freely moving mice.
3：List of Experimental Equipment and Materials:
Tapered optical fibers, femtosecond-pulsed near-infrared laser, photomultiplier tubes (PMTs), galvanometric scan system, fluorescence filters, and custom software for data acquisition and analysis.
4：Experimental Procedures and Operational Workflow:
Characterized light collection properties of TFs in fluorescent solutions and brain slices. Performed in vivo experiments to monitor dopamine transients in freely moving mice.
5：Data Analysis Methods:
Analyzed light collection and photometry efficiency fields, and performed statistical comparisons between TFs and FFs.
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fluorescence filters
FF01-520/70-25
Semrock
Filtering out specific wavelengths of light for fluorescence detection.
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femtosecond-pulsed near-infrared laser
Chameleon Discovery
Coherent
Used for two-photon excitation in fluorescence generation.
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photomultiplier tubes
H10770PA-40
Hamamatsu
Detection of fluorescent light.
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tapered optical fibers
OptogeniX
Enable light collection over an extent of up to 2 mm of tissue and multisite photometry along the taper.
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galvanometric scan system
Sutter
Used for scanning the laser beam in the experimental setup.
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