研究目的
This study investigated effects of narrow-range ultraviolet irradiation (UVR) by a new UV–LED device on vitamin D supply and changes of bone in senescence-accelerated mouse P6 (SAMP6) with vitamin D deficiency.
研究成果
Long-term UVR delivered by the narrow-range UV–LED device is effective on 1,25(OH)2D supply as well as 25(OH)D in SAMP6 mice with vitamin D deficiency. Furthermore, this UVR treatment may have also possibility to improve bone metabolism associated with vitamin D deficiency in SAMP6 mice. UVR treatment with a UV–LED device may represent a promising novel therapeutic approach to this disease.
研究不足
1. Only serum 25(OH)D and 1,25(OH)2D levels were measured due to small blood sample volume. 2. The sample size was relatively small. 3. No group of mice fed with a vitamin D-containing diet with UVR was included. 4. The protocol used may not be directly adaptable to clinical use due to high UVR dose and harmful wavelength.
1：Experimental Design and Method Selection:
Female SAMP6 mice were used as a senile osteoporotic model. Mice were divided into three groups: D-UVRt (vitamin D deficient–dietary and UVR), D- (vitamin D deficient–dietary), and Dt (vitamin D contained–dietary). Mice in the D-UVRt group were UV–irradiated (305nm) with 1 kJ/m2 twice a week for 12 weeks from 20 to 32 weeks of age.
2：Sample Selection and Data Sources:
Serum 25(OH)D, 1,25(OH)2D, and micro–computed tomography (CT) were assessed over time. Mechanical test and histological assay were performed for femurs removed at 32 weeks of age.
3：List of Experimental Equipment and Materials:
UV–LED device emitting UVB at a 305 nm wavelength, micro–CT scanner (SkyScan 1176), materials testing machine (MZ500D).
4：Experimental Procedures and Operational Workflow:
UV irradiation was performed twice a week for 12 weeks. Blood samples were collected at specific time points for serum analysis. Micro–CT scans were performed every 4 weeks. Mechanical tests and histological assays were conducted on femurs at the end of the study.
5：Data Analysis Methods:
Serum levels of 25(OH)D and 1,25(OH)2D were measured using RIA. Micro–CT data were analyzed for trabecular and cortical bone parameters. Mechanical parameters were calculated from load–displacement curves. Histological assays included TRAP staining and Masson's trichrome staining.
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