研究目的
To detect DNA with high sensitivity using surface enhanced Raman spectroscopy (SERS) by applying dye labeled oligonucleotides and determining the optimal distance between the SERS label and the silver surface for strong signal enhancement.
研究成果
The study successfully applied silver nanoparticles and dye labeled oligonucleotides for DNA detection with high sensitivity, achieving a limit of detection of 3 fmol dm–3 for Rh6G labeled oligonucleotides. The optimal distance between the SERS label and AgNPs was determined to be crucial for signal enhancement.
研究不足
The sensitivity of detection varies with the distance between the dye and AgNPs, and the method requires optimization for different dyes and oligonucleotides.
1：Experimental Design and Method Selection:
Utilized surface enhanced Raman spectroscopy (SERS) with dye labeled oligonucleotides for DNA detection.
2：Sample Selection and Data Sources:
Used oligonucleotides labeled with Rh6G or Cy3 at the 5' end, complementary to different regions of oligonucleotides bound to AgNPs.
3：List of Experimental Equipment and Materials:
Silver nanoparticles (AgNPs) as SERS signal enhancers, Rh6G and Cy3 labeled oligonucleotides.
4：Experimental Procedures and Operational Workflow:
Hybridization of target DNA with oligonucleotides immobilized on solid phase, followed by selective conjugation with AgNPs.
5：Data Analysis Methods:
Measured Raman signal intensity to determine the optimal distance between dyes and AgNPs for signal enhancement.
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