研究目的
To provide students with hands-on experience in identifying proteins using four methods of MALDI-TOF MS and to emphasize the importance of sequencing proteins/peptides in proteomic research.
研究成果
The experiment successfully introduces students to standard proteomic techniques and a variety of MALDI methods, preparing them for advanced experiments and research projects in systems biology.
研究不足
The experiment requires access to a MALDI-TOF MS instrument, which may not be available in all institutions. Careful scheduling of individual access sessions for students is necessary due to the singularity of instrument access.
1：Experimental Design and Method Selection:
Students perform four MALDI methods (linear positive, in-source decay, peptide mass fingerprint, and postsource decay) on four proteins.
2：Sample Selection and Data Sources:
Bovine serum albumin, equine myoglobin, bovine calmodulin, and bovine cytochrome c are used as samples.
3：List of Experimental Equipment and Materials:
MALDI-TOF MS instrument, trypsin, dithiothreitol, iodoacetamide, and other reagents.
4：Experimental Procedures and Operational Workflow:
Students reduce proteins, protect thiols, digest with trypsin, and perform MALDI-TOF MS analysis.
5：Data Analysis Methods:
Students analyze MS data using software tools to identify proteins.
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