摘要： Blood-contacting medical devices that directly inhibit thrombosis and bacterial infection without using dangerous anticoagulant and antibacterial drugs can save countless lives but have proved extremely challenging. Here, a useful methodology is proposed that employs temporally shaped femtosecond laser ablation combined with fluorination to fabricate a multifunctional three-dimensional (3D) micro-nanostructures with excellent hemocompatibility, zero cytotoxicity, outstanding biocompatibility, bacterial infection prevention, and long-term effectiveness on NiTi alloys. This multifunctional 3D micro-nanostructures presents 0.1% hemolysis ratio, almost no platelet adhesion and activation, repels blood to inhibit blood coagulation in vitro, maintains 100% cell viability and has exceptional stability over 6 months. Moreover, the multifunctional 3D micro-nanostructures simultaneously suppresses bacterial colonization to form biofilm and kills 100% colonized P. aeruginosa and 95.6% colonized S. aureus after 24h incubation, and bacterial residues can be easily removed. The fabrication method in this work has the advantages of simple processing, high efficiency, high quality and high repeatability, and the new multifunctional 3D micro-nanostructures can effectively prevent thrombosis and bacterial infection, which can be widely applied to various clinical needs such as biomedical devices and implants.