研究目的
Investigating molecular energy metabolism of single cells, especially cancer cells, during hyperthermia cell death process to better understand the photothermal lethal mechanism of cancer cells and design new photothermal probes more rationally.
研究成果
The study successfully monitored the ATP content changes in single cells during hyperthermia cell death using a turn-on type fluorescent nanoprobe. It was found that the increase in ATP content in cancerous cells was notably higher than in normal cells during the hyperthermia cell death, providing insights into the energy metabolism differences between cancerous and normal cells under photothermal stimulation.
研究不足
The study focuses on the ATP content changes during hyperthermia cell death but does not explore the detailed mechanisms behind the observed differences in ATP production between cancerous and normal cells. The photothermal therapy's efficacy and the nanoprobes' biocompatibility in vivo were not investigated.
1：Experimental Design and Method Selection:
The study involved the synthesis of a nuclear targeting photothermal probe (AuNRs@NLS) for executing AuNRs-PPTT and preparing a turn-on type fluorescent nanoprobe (ATP nanoflares) based on aptamer recognition to investigate the ATP metabolism of cells during hyperthermia cell death.
2：Sample Selection and Data Sources:
HeLa and H8 cells were used to examine the ATP metabolism response difference between cancerous cells and normal cells when exposed to photothermal stimulation.
3：List of Experimental Equipment and Materials:
Gold nanorods (AuNRs), ATP-specific nucleic acid aptamer, Cy3 labeled complementary reporter DNA, 808 nm laser.
4：Experimental Procedures and Operational Workflow:
Cells were treated with AuNRs@NLS and ATP nanoflares, then irradiated with 808 nm laser at a density of 2 W cm-2 for different times to monitor ATP content fluctuations.
5：Data Analysis Methods:
Fluorescence intensity was measured to monitor ATP content changes in cells during the hyperthermia cell death process.
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