研究目的
Investigating the therapeutic effects of a biomineralization-inspired nanozyme for single-wavelength laser activated photothermal-photodynamic synergistic treatment against hypoxic tumors.
研究成果
RBIR nanozyme demonstrated enhanced therapeutic outcomes against hypoxic tumors through synergistic photothermal and photodynamic therapy under single-wavelength laser activation, with negligible side effects. The simple synthetic route and imaging capabilities make RBIR a promising agent for clinical applications.
研究不足
The study focuses on the in vitro and in vivo efficacy of RBIR against hypoxic tumors, but the long-term stability, biodistribution, and potential toxicity of RBIR in humans require further investigation.
1：Experimental Design and Method Selection:
The study employed a biomineralization strategy to prepare a catalase-like nanozyme RuO2@BSA (RB) and loaded it with a near-infrared photosensitizer (IR-808-Br2) to generate RuO2@BSA@IR-808-Br2 (RBIR) for enhanced phototherapy against hypoxic tumors.
2：Sample Selection and Data Sources:
4T1 tumor-bearing mice and 4T1 cells were used to evaluate the therapeutic efficacy and mechanism of RBIR.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM), dynamic light scattering (DLS), inductively coupled plasma-atomic emission spectroscopy (ICP-AES), UV-Vis spectroscopy, energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), circular dichroism (CD), fluorescence microscopy, and a non-contact thermal imaging system were used.
4：Experimental Procedures and Operational Workflow:
RBIR was synthesized, characterized, and its therapeutic efficacy was evaluated in vitro and in vivo through photothermal and photodynamic therapy under single-wavelength laser activation.
5：Data Analysis Methods:
The data were analyzed using various spectroscopic and imaging techniques to assess the therapeutic outcomes and mechanisms of RBIR.
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Transmission electron microscope
Characterization of the physical/chemical properties of RBIR
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Dynamic light scattering
Particle size distribution measurement of RBIR
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Inductively coupled plasma-atomic emission spectroscopy
Determination of the mass ratio of RuO2 to BSA
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UV-Vis spectroscopy
Determination of the mass ratio of IR-808-Br2 to RB
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Energy-dispersive X-ray spectroscopy
Confirmation of the existence of ruthenium and bromine in RBIR
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X-ray photoelectron spectroscopy
Determination of the oxidation state of ruthenium in RBIR
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Circular dichroism
Assessment of the α-helix structure retention in RBIR
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Fluorescence microscopy
Evaluation of intracellular molecular oxygen levels and HIF-1α level
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Non-contact thermal imaging system
Monitoring of the body temperature change profiles during phototherapy
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