研究目的
Investigating the structural changes of CL-bound Fe2+Cyt c and the correlation with Cyt c release through surface-enhanced Raman spectroscopy (SERS) on nickel substrates to understand the role of the ferrous state of Cyt c in triggering apoptosis.
研究成果
The study revealed that the ferrous state of Cyt c plays a more significant role in triggering apoptosis by inducing higher peroxidase activity and membrane permeability upon binding with CL, as evidenced by SERS and molecular dynamics simulations.
研究不足
The study focuses on the in vitro interaction between Cyt c and CL on nickel substrates, which may not fully replicate the complex in vivo environment of mitochondria.
1：Experimental Design and Method Selection:
Utilized SERS on nickel substrates to study the interaction between Cyt c and CL. Molecular dynamics simulation was conducted to explore conformational changes.
2：Sample Selection and Data Sources:
Prepared CL liposomes to mimic the structure of lipid bilayers in the inner mitochondrial membrane.
3：List of Experimental Equipment and Materials:
Nickel nanostructures as SERS-active substrates, CL liposomes, and Cyt c.
4：Experimental Procedures and Operational Workflow:
Incubated Cyt c with CL liposomes, adsorbed onto Ni substrates, and measured SERS spectra.
5：Data Analysis Methods:
Analyzed SERS spectra to determine structural changes and peroxidase activity.
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