1：Experimental Design and Method Selection:

The study involved analyzing the expression of CD49a on dNK cells from patients with recurrent spontaneous abortions and healthy controls using flow cytometry. DNK cells were treated with CD49a neutralizing antibody to analyze their function. LncRNA microarray analysis was used to identify a potential regulator of CD49a.

2：9a. Sample Selection and Data Sources:

2. Sample Selection and Data Sources: Decidual tissues were obtained from healthy women undergoing voluntary medical abortion and women with a history of recurrent spontaneous abortions.

3：List of Experimental Equipment and Materials:

NK cell Isolation Kit, Human NK Cell Nucleofector Kit, neutralizing antibody reactive with CD49a, various antibodies for flow cytometry, Fixation/Permeabilization Solution Kit, Fibronectin, Ficoll‐Hypaque, Collagenase IV.

4：Experimental Procedures and Operational Workflow:

Separation of decidual mononuclear cells, isolation of NK cells, flow cytometric detection of dNK cell surface molecules, [51Cr] release assay for measurement of NK cell killing, quantitative real‐time polymerase chain reaction (qRT‐PCR) analysis, design, synthesis, and nuclear transfection of Lnc‐49a siRNA, Transwell assay, adhesion assay.

5：Data Analysis Methods:

Statistical analysis was performed by GraphPad‐Prism‐6 using two‐tailed t tests to check statistical value of the differences between groups.