研究目的
To develop a highly selective detection method for glutathione (GSH) using amino-rich graphene quantum dots (GQDs) and copper ions (Cu2+) as a fluorescence switch.
研究成果
The developed method using GQDs and Cu2+ as a fluorescence switch provides a highly selective and sensitive approach for detecting GSH in cell lysate, with potential applications in biomedical research and diagnostics.
研究不足
The method's sensitivity and selectivity may be affected by the presence of other thiol compounds and the medium's acidity. The fluorescence of GQDs is relatively low under acidic conditions.
1：Experimental Design and Method Selection:
The study utilized the coordination between GQDs and Cu2+ to induce fluorescence quenching, and the stronger coordination of GSH with Cu2+ to recover fluorescence.
2：Sample Selection and Data Sources:
GQDs were synthesized from Ginkgo biloba leaves. GSH and other biomolecules were purchased from Sigma-Aldrich.
3：List of Experimental Equipment and Materials:
Instruments included a fluorescence spectrophotometer, UV spectrophotometer, TEM, AFM, XPS, FTIR spectrometer, XRD, fluorescence spectrometer, and a microplate reader. Materials included GSH, Cys, His, CuSO4, and other reagents.
4：Experimental Procedures and Operational Workflow:
GQDs were synthesized, characterized, and used in GSH detection experiments under optimized conditions.
5：Data Analysis Methods:
Fluorescence intensity changes were measured and analyzed to determine GSH concentration.
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F-2500 fluorescence spectrophotometer
F-2500
Hitachi
Measuring fluorescence spectra of GQDs
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UV-3600 spectrophotometer
UV-3600
Shimadzu
Obtaining absorption spectra of GQDs
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High resolution transmission electron microscopy
Tecnai G2 F20 S-TWIN
FEI Company
Taking electron microscopy images of GQDs
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Fourier infrared spectrometer
FTIR-8400S
Shimadzu
Analyzing the surface functional groups of GQDs
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X-ray diffractometer
XRD-7000
Shimadzu
Analyzing the crystal structure of GQDs
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Absolute PL quantum yield spectrometer
C11347
Hamamatsu
Determining the quantum yield of GQDs
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Atomic force microscopy
ESCALAB 250Xi
AFM Thermo
Analyzing the morphology of GQDs
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X-ray photoelectron spectroscopy
ESCALAB 250
Murfisher scientific company
Analyzing the elements and surface functional groups of GQDs
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Fluorescence spectrometer
FL-TCSPC
Horiba jobin Yvon Inc
Determining the fluorescence lifetime of GQDs
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Synergy H1 multi-functional microplate reader
Synergy H1
Berton
Evaluating the toxicity of GQDs to cells
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Universal electronic furnace
DL-1
Beijing Ever Bright Medical Treatment Instrument Co., Ltd.
Synthesizing GQDs
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Microwave oven
P70D20P-NPCWO
Galanz
Synthesizing GQDs
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Centrifuge
Shanghai Anting Scientific Instrument Factory
Purifying GQDs
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