研究目的
To develop a method for creating ultra-stable plasmonic colloidal aggregates for accurate and reproducible quantitative SE(R)RS in protein-rich biomedia.
研究成果
The emulsion templated method allows for the reproducible production of ultra-stable SE(R)RS active colloidal aggregates, significantly improving the stability and reproducibility of SE(R)RS measurements in complex systems like protein-rich bio-solutions.
研究不足
The method's applicability is limited to the types of colloidal NPs used and may require optimization for different materials or conditions.
1：Experimental Design and Method Selection:
The study employs an emulsion templated method to form densely-packed quasi-spherical Au/Ag colloidal aggregates.
2：Sample Selection and Data Sources:
Citrate-reduced Ag and Au colloids were used.
3：List of Experimental Equipment and Materials:
Silver nitrate, tetrachloroauric (III) acid trihydrate, trisodium citrate dihydrate, tetrabutylammonium nitrate, hexane, adenine, phenytoin, magnesium sulphate, albumin, and crystal violet were purchased from Sigma Aldrich. A Soniprep 150 Ultrasonic Disintegrator was used for emulsification.
4：Experimental Procedures and Operational Workflow:
The colloid was emulsified with hexane and TBA+NO3-, then left to allow complete evaporation of hexane to produce stable SPA colloids.
5：Data Analysis Methods:
SE(R)RS measurements were performed using a WITec Alpha 300R Confocal Raman Microscope.
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