研究目的
To establish a universal and sensitive plasmonic sensing strategy for biomolecule assays by coupling the hybridization chain reaction (HCR) strategy and a triple-helix molecular switch.
研究成果
The developed plasmonic sensing strategy is versatile, sensitive, and specific for the detection of various biomolecules. It offers a simple, cost-effective, and enzyme-free approach for biomarker detection with potential applications in disease diagnosis and drug screening.
研究不足
The method may require optimization for different targets and matrices. The sensitivity and specificity may vary depending on the target and the complexity of the sample matrix.
1：Experimental Design and Method Selection:
The strategy involves the use of a triple-helix molecular switch (THMS) to release a universal trigger (UT) upon target recognition, which then initiates the HCR process between two hairpins (M1 and M2). This leads to the aggregation of gold nanoparticles (AuNPs) and a change in absorbance at 521 nm.
2：2). This leads to the aggregation of gold nanoparticles (AuNPs) and a change in absorbance at 521 nm. Sample Selection and Data Sources:
2. Sample Selection and Data Sources: The method was tested with nucleic acids (miRNA-21), small molecules (ATP), and proteins (thrombin) as targets.
3：List of Experimental Equipment and Materials:
Gold nanoparticles (AuNPs), hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O), tris(2-carboxyethyl) phosphine hydrochloride (TCEP), sodium citrate, and oligonucleotides were used.
4：Experimental Procedures and Operational Workflow:
The THMS was constructed in an aqueous solution, and the HCR process was initiated by the released UT. The aggregation of AuNPs was monitored by UV-Vis spectroscopy.
5：Data Analysis Methods:
The change in absorbance at 521 nm was used to quantify the target concentration.
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