研究目的
Investigating the development of a sensitive sensor for detecting chlorothalonil (CHL) based on the inner-filter effect (IFE) between gold nanoparticles (AuNPs) and ratiometric fluorescent quantum dots (RF-QDs).
研究成果
The study successfully developed a ratiometric fluorescence sensor for detecting CHL with high sensitivity, wide linear range, good stability, and selectivity. The sensor's practical application was demonstrated in food and environmental samples, showing satisfactory results and good repeatability.
研究不足
The study did not explore the long-term stability of the sensor in real-world environmental conditions or its performance in the presence of a wide range of interfering substances beyond those tested.
1：Experimental Design and Method Selection:
The study involved the design of RF-QDs by two different color CdTe QDs and the use of AuNPs to quench the fluorescence of the RF-QDs based on the IFE. The electrostatic attraction between protamine (PRO) and AuNPs was utilized to restore fluorescence, and the hydrolysis of PRO by papain (PAP) was used to quench fluorescence again. The inhibition of PAP activity by CHL was studied to restore the fluorescent signal.
2：Sample Selection and Data Sources:
The study used RF-QDs and AuNPs as the main materials, with CHL as the target analyte.
3：List of Experimental Equipment and Materials:
The materials included CdTe QDs, AuNPs, PRO, PAP, and CHL. The equipment included a fluorescent spectrophotometer, UV-visible absorption spectrometer, Zetasizer Nano ZS, precision pH meter, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and circular dichroism (CD) analysis apparatus.
4：Experimental Procedures and Operational Workflow:
The procedure involved the preparation of CdTe QDs, RF-QDs, and AuNPs, followed by the detection of CHL using the PAP/PRO/AuNPs/RF-QD system.
5：Data Analysis Methods:
The fluorescence spectra were analyzed to evaluate the inhibition of PAP activity by CHL and the detection of CHL in samples.
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Fluorescent spectrophotometer
FluoroMax-4
HORIBAI
Testing fluorescence spectra
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Ultraviolet spectrophotometer
Cary 60 UV-Vis
Agilent
Obtaining UV-visible absorption spectrum
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Zetasizer Nano ZS
UK
Testing zeta potentials
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Precision pH meter
CTARTER 3100
USA
Monitoring and adjusting pH
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Transmission electron microscopy
JEOL-2100F
Japan
Obtaining TEM images
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Fourier transform infrared spectroscopy
Lambda 950
USA
Obtaining FTIR images
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Circular dichroism
DSM 1000
USA
Obtaining CD analysis images
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