研究目的
Examining plasmonic thermal destruction of murine melanoma using gold nanoparticles obtained by green chemistry and comparing its antitumor effect with the clinical reference treatment: anti-PD1 monoclonal antibody.
研究成果
The study demonstrated the potential of plasmonic photothermal therapy using gold nanoparticles as a non-invasive alternative treatment for melanoma. The treatment showed antitumor activity with minimal toxicity, comparable to the reference treatment (anti-PD1 monoclonal antibody). Further research is recommended to optimize laser power density for enhanced efficacy.
研究不足
The study was conducted on a murine model, which may not fully replicate human melanoma. The power density of the laser was low (0.2W/cm2), which might limit the efficacy of the treatment. Further studies are needed to test intermediate power lasers.
1：Experimental Design and Method Selection:
The study involved measuring tumor volume and mice weight in different groups of mice with murine melanoma B16F10, treated or not with gold nanoparticles and coupled to laser irradiation. The data were compared to the clinical reference treatment: anti-PD1 monoclonal antibody.
2：Sample Selection and Data Sources:
25 melanoma bearing mice divided into 5 groups were used. Groups included injection of nanoparticles without laser, laser exposure alone, nanoparticles combined with laser, and a reference treatment group (anti-PD1 monoclonal antibody).
3：List of Experimental Equipment and Materials:
Gold nanoparticles (NP@G@P), digital caliper for tumor measurements, scale for weight measurements, 808 nm laser diode for irradiation.
4：Experimental Procedures and Operational Workflow:
Mice were subcutaneously engrafted with B16F10 murine melanoma cells. Treatment started when tumor volume reached 120 mm3. Mice were weighed and tumor volume measured every 3 days until death or sacrifice.
5：Mice were weighed and tumor volume measured every 3 days until death or sacrifice.
Data Analysis Methods:
5. Data Analysis Methods: Tumor volume was calculated using the formula: Volume = (width x length x thickness)/2. Body weight changes and tumor growth inhibition were analyzed to assess treatment efficacy and toxicity.
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