研究目的
To establish the repeatability of baseline diagnostic images of the dorsum of the hands acquired using a high resolution Laser Doppler imager in patients with Raynaud’s phenomenon secondary to Systemic Sclerosis (SSc).
研究成果
High resolution laser Doppler imaging is a repeatable imaging modality for acquiring baseline images of the dorsal side of hands and assessing average flux values across the fingers, thumbs, and distal dorsal differences in patients with Raynaud’s phenomenon secondary to SSc. However, a larger study is required to confirm these findings due to the limitations of the sample size.
研究不足
The study was limited by a small sample size (19 patients analyzed out of 22 recruited), potential variations in patients' health between consecutive days, and the impact of external environmental conditions on the results. Technical issues and patient movement also affected some data.
1：Experimental Design and Method Selection:
The study involved imaging the dorsal side of the hands of patients with SSc and secondary Raynaud’s phenomenon over two consecutive days using a Moor Instruments High Resolution Laser Doppler imaging unit. The images were analyzed by taking regions of interest at discrete locations to calculate dimensionless values of flux (PU). Repeatability was assessed using methods described by Bland and Altman and by plotting results from visit 1 against visit 2 to calculate the line of best fit.
2：Sample Selection and Data Sources:
22 patients (8 male, 14 female) with SSc and secondary Raynaud’s phenomenon, age range 29-73, median 62, were included. Patients were stable on vasoactive medication and refrained from drinking caffeinated tea and coffee 4 hours before the investigations.
3：List of Experimental Equipment and Materials:
Moor Instruments High Resolution Laser Doppler imaging unit (moorLDI2-IR), Tiny Tag Talk 2 data logger for room temperature measurement, FLIR SC305 thermal imaging camera for hand temperature assessment.
4：Experimental Procedures and Operational Workflow:
Patients were imaged at approximately the same time on two consecutive days. The room temperature was maintained at approximately 25°C. The hands were placed on a black neoprene mat, and images were acquired at scan resolutions of 85 on the y-axis and 200 to 230 on the x-axis, with a sample time for each pixel set to 4ms.
5：Data Analysis Methods:
Images were analyzed using Moor Review software V6.0 to create regions of interest and calculate mean flux values. Repeatability was assessed using Bland & Altman methods and scatter plots with lines of best fit.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
