研究目的
Investigating the therapeutic effects of gold nanoparticles doped iron-based metal-organic frameworks (GIM) as near-infrared light-enhanced cascade nanozyme against hypoxic tumors.
研究成果
The study demonstrates that GIM, as a NIR light-enhanced cascade nanozyme, can effectively protect the GOx-like activity of AuNPs, enhance ROS generation at tumor sites, and significantly inhibit tumor growth in vivo. This strategy offers a promising approach for tumor catalytic therapy, with potential for broader application in engineering other nanozymes with external energy field stimulation.
研究不足
The study primarily focuses on the in vitro and in vivo evaluation of GIM's therapeutic effects, with limited discussion on potential side effects or long-term biocompatibility. The scalability and clinical applicability of GIM synthesis and application require further investigation.
1：Experimental Design and Method Selection
The study involved the synthesis of gold nanoparticles doped iron-based metal-organic frameworks (GIM) and evaluation of its near-infrared light (NIR)-enhanced cascade nanozyme activity against hypoxic tumors. The methodology included the synthesis of GIM, characterization of its properties, and assessment of its therapeutic effects in vitro and in vivo.
2：Sample Selection and Data Sources
The samples included GIM and its components (AuNPs and iron-based MOFs). Data sources included in vitro cell culture experiments (A549 and LO2 cell lines) and in vivo tumor xenograft models in female Blab/c nude mice.
3：List of Experimental Equipment and Materials
Materials included FeCl2·4H2O, H3BTC, HAuCl4, NaBH4, DMPO, TMB, PTA, DCFH-DA, [Ru(dpp)3]Cl2, and others. Equipment included TEM, EDX, FT-IR, XRD, XPS, UV–vis spectrometer, fluorescence spectrometer, EPR spectrometer, infrared thermal imaging camera, and ICP-MS.
4：Experimental Procedures and Operational Workflow
The experimental procedures involved the synthesis of GIM, characterization of its catalytic performance, assessment of its photothermal effect under NIR light irradiation, evaluation of its therapeutic effects in vitro and in vivo, and analysis of its biodistribution and metabolism in vivo.
5：Data Analysis Methods
Data analysis methods included UV–vis spectroscopy, fluorescence spectroscopy, EPR spectroscopy, flow cytometry, and ICP-MS for quantitative analysis of Au ion concentrations in tissues and excrement.
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TEM
Characterization of IM and GIM morphology
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EDX
Elemental analysis of GIM
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FT-IR
Characterization of GIM
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XRD
Characterization of GIM
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XPS
Characterization of GIM
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UV–vis spectrometer
Absorbance spectrum measurement
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Fluorescence spectrometer
Fluorescence spectra measurement
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EPR spectrometer
Detection of (cid:1668)OH
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Infrared thermal imaging camera
Testo 865
Testo Instrument international trading Co. Ltd., China
Temperature recording
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ICP-MS
Analysis of Au ion concentrations
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