研究目的
To study the changes in the secondary structure of proteins of biological fluids of the oral cavity by synchrotron infrared microscopy during the development of cariosity in deep dentin tissues.
研究成果
The development of pathological processes of caries in dentin is reflected in the composition of biological fluids of the oral cavity. Certain changes in the secondary structure of the protein of biological fluids are reliable spectroscopic signatures of pathology and can be easily detected without laborious and redundant extraction of dentinal fluid, since they are also present in the gingival fluid, the extraction of which for screening is a less difficult procedure.
研究不足
The study is limited by the sample size and the specific age group of participants. The complexity and mere impossibility of in vivo extraction of the dentinal fluid is a main obstacle in the development of a new diagnostic method.
1：Experimental Design and Method Selection:
The study involved ten people (five men and five women) from 22 to 28 years of age. Three samples of biological fluids were taken from each patient with detected dentin caries, specifically, the dentinal fluid, the fluid from the gingival sulcus, and the gingival blood. The molecular compositions of the samples were studied using the IR spectroscopy technique on the equipment of the infrared microspectroscopy (IRM) beamline at the Australian Synchrotron and a Bruker Vertex 80V spectrometer with a detector cooled by liquid nitrogen.
2：Sample Selection and Data Sources:
The biological fluids of the oral cavity were sampled using a specialized vacuum setup and original microcapillaries filled with homogenized KBr powder. After sampling, the KBr powder from microcapillaries containing the biological fluids was dried at room temperature.
3：List of Experimental Equipment and Materials:
Bruker Vertex 80V spectrometer, infrared microspectroscopy (IRM) beamline at the Australian Synchrotron, specialized vacuum setup, microcapillaries, KBr powder.
4：Experimental Procedures and Operational Workflow:
The samples were analyzed using IR spectroscopy to study the molecular composition. The amide I and amide II bands were decomposed into components by means of Gaussian curves to identify changes in the secondary structure of proteins.
5：Data Analysis Methods:
The spectra were decomposed into components according to a method that has been proposed and tested in a number of studies, taking into account the imposed restrictions and critical remarks regarding the calculation of the number of maxima in the spectrum, the drawing of the base line, and the determination of the convergence of the decomposition result.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
