研究目的
To develop a low-cost, portable, and user-friendly paper-based analytical device for simultaneous determination of orthophosphate and calcium ions in human serum for hyperphosphatemia diagnostics.
研究成果
The developed LED&Paper-based analytical device is a low-cost, portable, and user-friendly tool for simultaneous determination of orthophosphate and calcium ions in human serum. It offers high sensitivity and low detection limits, making it suitable for point-of-care diagnostics. The device's practical utility was successfully demonstrated using control serum standards and real human serum samples.
研究不足
The measurements were performed under laboratory conditions without special control of temperature and humidity. The device's precision may be affected by the inhomogeneity of paper and the precision of solid ink printing.
1：Experimental Design and Method Selection:
The device was designed for simultaneous detection of orthophosphate and calcium ions using fluorescence detection. The paper-based device was prepared by wax printing on filter paper, creating a 'sandwich-type' detection zone for phosphate ions and a single circle for calcium ions.
2：Sample Selection and Data Sources:
Control serum standards and real human serum samples were used. Samples were diluted to fit the linear ranges of the device.
3：List of Experimental Equipment and Materials:
Whatmann qualitative filter paper, wax printer, LEDs (525 nm green, 655 nm red, 470 nm blue, 630 nm red), multimeter, and reagents (Rhodamine B, calcein, ammonium heptamolybdate, etc.).
4：Experimental Procedures and Operational Workflow:
Samples were deposited on the paper strip, which transported the solution to detection zones via capillary action. Fluorescence signals were measured after drying.
5：Data Analysis Methods:
Calibration graphs were constructed for both ions, and statistical analysis was performed to compare results with reference methods.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
