研究目的
Investigating the therapeutic effects of a specific herbal medicine on a particular disease.
研究成果
The study successfully developed a biocompatible, pH-sensitive fluorescent gold nanocluster (PSP-Y-AuNCs) with high quantum yield for real-time tumor imaging. The PSP-Y-AuNCs demonstrated excellent biocompatibility, efficient cellular uptake, and specific tumor targeting due to the acidic microenvironment of tumors. The findings suggest that PSP-Y-AuNCs hold great promise as a non-toxic, biocompatible imaging probe for cancer diagnosis and monitoring.
研究不足
The study primarily focuses on the fabrication and initial biocompatibility and imaging applications of PSP-Y-AuNCs. Further detailed studies on the long-term toxicity, pharmacokinetics, and therapeutic efficacy in more complex tumor models are needed to fully understand its potential clinical applications.
1：Experimental Design and Method Selection:
The study involved the isolation of a biocompatible natural polysaccharide (PSP001) from the fruit rind of Punica granatum, conjugation with L-cysteine (Y), and fabrication of fluorescent gold nanoclusters (AuNCs).
2：Sample Selection and Data Sources:
Human red blood cells (RBCs), human lymphocytes, normal human keratinocytes (HaCaT), human lung adenocarcinoma (A549), and mouse breast cancer (4T1) cells were used for biocompatibility and cellular internalization studies. BALB/c mice and NCr nude female mice were used for in vivo toxicity and imaging studies.
3：List of Experimental Equipment and Materials:
Tetrachloroauric acid (HAuCl4), NaBH4, L-Cysteine, Nigercin sodium salt, MTT, DMEM, antibiotic-antimycotic mixture, and various chemicals for buffer preparation.
4：Experimental Procedures and Operational Workflow:
Synthesis of PSP-Y-AuNCs involved conjugation of PSP001 with Y, followed by fabrication of AuNCs under specific pH conditions. Characterization was done using UV-visible spectroscopy, fluorescence spectroscopy, HRTEM, zeta potential measurement, FTIR, and ICP-AES. Biocompatibility was assessed through hemolysis assay and cell viability assays. Cellular internalization was studied using confocal microscopy and flow cytometry. In vivo imaging was performed using an IVIS imaging system.
5：Data Analysis Methods:
Fluorescence intensity and pH ratiometric imaging were analyzed using ImageJ software. Total radiant efficiency and average radiant efficiency were calculated using Living Image Software.
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