研究目的
To find an approach for effective skin optical clearing in vivo using polyethylene glycol 300 (PEG-300) as an optical clearing agent in combination with physical enhancers: fractional laser microablation (FLMA), and/or low-frequency sonophoresis.
研究成果
The combination of FLMA, PEG-300, and sonophoresis was the most effective approach for skin optical clearing in the upper dermis, achieving the highest optical clearing potential in 30 min. Sonophoresis with PEG-300 was the most effective for the middle dermis, but required 90 min to achieve maximal optical clearing potential.
研究不足
The study was limited to albino outbred rats and may not fully represent human skin responses. The observation period was 90 min, which may not capture longer-term effects.
1：Experimental Design and Method Selection:
The study used albino outbred rats to evaluate the light attenuation coefficient and optical clearing potential of PEG-300 in combination with FLMA and/or low-frequency sonophoresis in upper and middle dermis separately using OCT.
2：Sample Selection and Data Sources:
Twenty albino outbred rats were separated into four groups for different treatments.
3：List of Experimental Equipment and Materials:
PEG-300 (Sigma-Aldrich, USA), StarLux/Lux2940 Er:YAG laser (Palomar Medical Technologies Inc., USA) with a Booster-2 module (Laser Centre, ITMO University, Russia), therapeutic ultrasound device Dinatron 125 (Dinatronics, USA), Thorlabs OCP930R spectral optical coherence tomography (Thorlabs, USA).
4：Experimental Procedures and Operational Workflow:
The animals were treated with PEG-300 alone, FLMA and then PEG-300, sonophoresis with PEG-300, and combination FLMA, PEG-300 and sonophoresis. Skin was monitored before any treatments and then every 5 min using OCT.
5：Data Analysis Methods:
The OCT signal was approximated using a single scattering model to evaluate the attenuation coefficient and optical clearing potential.
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