研究目的
Investigating the minimum bactericidal concentration (MBC) of the silver nanoparticles on two different bacteria cultures as well as the possibility to enhance their antimicrobial activity using laser irradiation.
研究成果
The antibacterial activity of Ag NPs against Escherichia coli and Staphylococcus aureus was tested with the MIC dilution methods. Results show that Ag NPs were effective against these pathogens. It was found that the concentration of silver nanoparticles in solution determines their bactericidal properties. Laser irradiation of nanoparticle solutions can enhance their bactericidal activity, especially for Staphylococcus aureus.
研究不足
The study did not investigate the influence of the irradiation time and radiation power on the bactericidal activity of the silver nanoparticles in detail.
1：Experimental Design and Method Selection:
The study used the MIC dilution method to determine the minimum bactericidal concentration of silver nanoparticles against Escherichia coli and Staphylococcus aureus. Laser irradiation was applied to enhance antimicrobial activity.
2：Sample Selection and Data Sources:
Silver nanoparticle colloid dispersion Metalon JS-B25HV (Novacentrix) was used. The size and morphology of AgNPs were determined by scanning electron microscopy.
3：List of Experimental Equipment and Materials:
A continuous wave diode laser was used for irradiation of silver nanoparticle colloids. Spectral width of the laser beam is
4：5 nm with the peak wavelength at 408 nm. Experimental Procedures and Operational Workflow:
A two-fold serial dilution of the original dispersion in liquid nutrient medium was performed. Bacteria were inoculated in the culture medium and kept in an incubator at 37 °С for 24 hours.
5：Data Analysis Methods:
The results were evaluated according to the presence or absence of microbial growth, performing visual inspection in transmitted light and comparing the degree of microbial turbidity of nutrient medium with the ?negative control?.
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