研究目的
To provide a simple, efficient, cost-effective and sensitive analytical method for quantification of deferiprone in plasma and validation according to FDA guidelines.
研究成果
The developed method based on GQDs and Fe3+ as a fluorescence probe is simple, low-cost, and efficient for determining the level of deferiprone in plasma. It shows the importance of GQDs for developing analytical methods for pharmaceuticals quantification.
研究不足
The method's sensitivity is 5 mg.L?1, which may not be sufficient for all applications. The study also mentions the complexity of plasma matrix which could affect fluorescence intensity.
1：Experimental Design and Method Selection:
A simple and cost-efficient methodology was developed utilizing the selective interaction of GQDs and deferiprone in the presence of Fe3+ ions. GQDs were synthesized by a bottom-up technique as a fluorescent probe.
2：Sample Selection and Data Sources:
Plasma samples were used, with deferiprone concentrations ranging from 5 to 50 mg.L?
3：List of Experimental Equipment and Materials:
UV-1800 Shimadzu spectrophotometer, JASCO FP-750 spectrofluorometer, FTIR-3400 Shimadzu, TEM (LEO 906, Zeiss), DLS (Malvern Instruments).
4：Experimental Procedures and Operational Workflow:
GQDs were synthesized, and their interaction with deferiprone in the presence of Fe3+ was studied. Fluorescence quenching was measured to quantify deferiprone.
5：Data Analysis Methods:
The calibration curve was generated, and method validation was performed according to FDA guidelines.
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