研究目的
Investigating the therapeutic effects of plasmonic nanoparticles irradiated by laser on benign prostatic hyperplasia in a rat model.
研究成果
The study demonstrates that laser-irritated NPs can be a novel and less invasive therapy for BPH, inducing apoptosis via caspase-1 pathway. Further research is needed to fully understand their therapeutic potential and safety.
研究不足
The study requires further investigation and characterization for complete understanding of the therapeutic potential of NPs. Toxicity and interaction of NPs with normal cells need more study.
1：Experimental Design and Method Selection:
The study involved the preparation and characterization of carbon nanotubes-polyethylene glycol (CNTs-PEG) for use in photothermal therapy.
2：Sample Selection and Data Sources:
Forty-eight adult male Sprague Dawley rats were used, divided into six groups for BPH induction and treatment.
3：List of Experimental Equipment and Materials:
Multi-walled carbon nanotubes (CNTs), polyethylene glycol (PEG), an 808 nm continuous-wave near-infrared laser diode, and various kits for RNA isolation, TUNEL assay, and TEM.
4：Experimental Procedures and Operational Workflow:
BPH was induced in rats, followed by treatment with NPs, laser, or a combination. Prostate size, PSA levels, histopathology, and apoptosis were assessed.
5：Data Analysis Methods:
Statistical analysis was performed using one-way ANOVA and Bonferroni post hoc test.
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polyethylene glycol
PEG1000
Sigma-Aldrich
Used to wrap carbon nanotubes for improved biocompatibility and functionality.
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multi-walled carbon nanotubes
CNTs
Plasmachem
Used in photothermal therapy for inducing hyperthermia in tumor cells.
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near-infrared laser diode
808-2W
Used to irradiate plasmonic nanoparticles for photothermal therapy.
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RNeasy mini kit
Qiagen
Used for RNA isolation from prostate tissues.
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Nanodrop 2000
Wilmington, Delaware, USA
Used to determine RNA purity.
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Applied Biosystems Real-Time PCR System
Life Technologies
Used for performing real-time PCR.
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Quantifast SYBR Green PCR kit
Qiagen
Used for real-time PCR according to the manufacturer’s instructions.
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transmission electron microscopy
Philips, 906E
Germany
Used to examine ultra-thin sections of prostate tissue.
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