研究目的
To realize an integrated opto-microfluidics platform for the detection of single droplet passage and estimation of its size without the need of any imaging processing, and to compare its performance with standard approaches.
研究成果
The LiNbO3-based platform presented high quality performances in terms of optical triggering, reproducibility and stability in time, allowing in real-time data analysis. It showed an increased capability better than 50% compared to standard approaches, paving the way for the realization of more complex, independent and portable LOC devices.
研究不足
The illuminated depth inside the channel is about 15-20 μm, which is 15-20% of the channel height, limiting the sensitivity to interfaces.
1：Experimental Design and Method Selection
The platform is based on the coupling of a self-aligned integrated optical stage, made of an array of optical waveguides, to a microfluidic circuit engraved in the same substrate. The fabrication involves photolithographic and thin-film deposition techniques for the waveguides and precision saw for the microfluidic channels.
2：Sample Selection and Data Sources
Commercial congruent LiNbO3 wafer was used as the substrate. Droplets of MilliQ? in a continuous phase of mineral oil with surfactant SPAN80? were generated and analyzed.
3：List of Experimental Equipment and Materials
He-Ne laser, precision saw with a diamond particle-polymeric blade, syringe pumps, photodiode, transimpedance amplifier, Agilent MSO-X 2012A oscilloscope.
4：Experimental Procedures and Operational Workflow
The optical trigger setup involved coupling a He-Ne laser into one waveguide, controlling light polarization and power, and analyzing the transmitted light across the microfluidic channel. Droplets were generated and their passage detected in real-time.
5：Data Analysis Methods
A customized software was developed to analyze the detected signal for the average signal value of each plateau and the passage time instant of droplets.
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