研究目的
To develop rapid and reliable sensor arrays based on tetraphenylethylene (TPE) derivatives for the detection and discrimination of pathogens, including normal and drug-resistant bacteria, with high accuracy.
研究成果
The developed sensor arrays based on TPE-AIEgens successfully identified various pathogens, including drug-resistant strains, with high accuracy. The method is rapid, simple, and does not require washing steps, making it suitable for clinical applications.
研究不足
The study focuses on a limited number of pathogens and may require validation with a broader range of clinical samples. The sensor arrays' performance in complex biological matrices was not extensively evaluated.
1：Experimental Design and Method Selection
The study designed sensor arrays using TPE-based AIEgens with cationic ammonium groups and different hydrophobic substitutions to enable multivalent interactions with pathogens. The fluorescence response patterns were analyzed using linear discriminant analysis (LDA).
2：Sample Selection and Data Sources
Seven microorganisms were used, including Gram-positive bacteria, Gram-negative bacteria, and fungi. Fluorescence intensities were recorded using a microplate reader.
3：List of Experimental Equipment and Materials
Materials included TPE derivatives, pathogens (S. aureus, E. faecalis, E. coli, P. aeruginosa, C. albicans), and solvents (DMSO, PBS). Equipment included a microplate reader, confocal laser scanning microscopy, and dynamic light scattering (DLS).
4：Experimental Procedures and Operational Workflow
Pathogens were incubated with TPE-ARs solutions, and fluorescence intensities were recorded. The process involved shaking, incubation, and fluorescence measurement without washing steps.
5：Data Analysis Methods
Fluorescence response patterns were analyzed using LDA for pathogen identification. The relative fluorescence intensities before and after incubation with pathogens were calculated.
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