研究目的
To test the hypothesis that blood–retinal barrier compromise is associated with the development of endogenous Staphylococcus aureus endophthalmitis.
研究成果
The results indicated that S. aureus is capable of inducing blood–retinal barrier permeability and causing endogenous bacterial endophthalmitis in normal and diabetic animals. The study supports the hypothesis that blood–retinal barrier compromise facilitates the development of endogenous bacterial endophthalmitis.
研究不足
The study primarily focuses on the murine model and in vitro human RPE cell culture, which may not fully replicate human physiological conditions. The specific mechanisms by which S. aureus induces blood–retinal barrier permeability require further investigation.
1：Experimental Design and Method Selection:
The study used a murine model of diabetes-associated endogenous bacterial endophthalmitis to assess the link between blood–retinal barrier compromise and the development of endogenous Staphylococcus aureus endophthalmitis. Streptozotocin-induced diabetes was induced in C57BL/6J mice for 1, 3, or 5 months. Diabetic and age-matched nondiabetic mice were intravenously injected with 108 colony-forming units (cfu) of S. aureus. After 4 days post infection, electroretinography, histology, and bacterial counts were performed.
2：Sample Selection and Data Sources:
Six-week-old C57BL/6J mice were used. Human ARPE-19 cells were propagated and maintained in Dulbecco’s modified Eagle’s medium (DMEM)/F
3：List of Experimental Equipment and Materials:
Equipment included electroretinography (ERG) recording system, confocal microscope, and fluorescence spectrophotometry. Materials included streptozotocin, S. aureus strain 8325-4, and FITC-dextran conjugates.
4：Experimental Procedures and Operational Workflow:
Mice were injected with S. aureus via the tail vein. Retinal function was measured by ERG. Eyes were harvested for bacterial quantitation or histology. In vitro, RPE monolayers were infected with S. aureus, and barrier function was assessed.
5：Data Analysis Methods:
Bacterial counts were determined by plating. ZO-1 immunopositivity was quantified using ImageJ. Statistical comparisons were made using Fisher’s exact test, Mann-Whitney U test, and t-tests.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
