研究目的
To review recent developments in molecular recognition approaches using atomic force microscopy (AFM) for studying cell surface receptors and their interactions with ligands, including cell-cell adhesion proteins, and to present examples of biological and biomedical applications.
研究成果
The combination of AFM imaging and force spectroscopy has expanded the capabilities of AFM beyond basic imaging, enabling the study of molecular recognition events on biological samples with high resolution. These methods have led to significant discoveries in cell biology, immunology, pharmacology, and medicine, and their continued development promises further advancements in these fields.
研究不足
The review highlights the technical challenges and limitations of AFM-based molecular recognition studies, such as the need for high spatial and temporal resolution, the complexity of sample preparation, and the interpretation of force spectroscopy data.
1：Experimental Design and Method Selection:
The review discusses two main approaches for combining AFM imaging and force spectroscopy: adhesion force mapping and topographic and recognition (TREC) imaging. These methods are used to study molecular recognition events on biological samples.
2：Sample Selection and Data Sources:
The review includes studies on various biological systems, such as microbial cells, human cells, and proteins, to demonstrate the applications of AFM in molecular recognition.
3：List of Experimental Equipment and Materials:
AFM is the primary equipment used, with specific modifications for force spectroscopy and imaging. Ligands are covalently attached to AFM tips for recognition studies.
4：Experimental Procedures and Operational Workflow:
Detailed procedures for adhesion force mapping and TREC imaging are described, including tip functionalization, sample preparation, and data acquisition.
5：Data Analysis Methods:
The review discusses the analysis of force-distance curves, generation of adhesion maps, and correlation of topographic and recognition images for molecular recognition studies.
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